Isolation and culture of embryonic stem-like cells from pig nuclear transfer blastocysts of different days.

@article{Tan2012IsolationAC,
  title={Isolation and culture of embryonic stem-like cells from pig nuclear transfer blastocysts of different days.},
  author={Guangyun Tan and Linzhu Ren and Yongye Huang and Xiaochun Tang and Yang Zhou and Yan Zhou and Dong Li and Hongxiao Song and Hongsheng Ouyang and Daxin Pang},
  journal={Zygote},
  year={2012},
  volume={20 4},
  pages={347-52}
}
This study was conducted to establish pig embryonic stem (ES)-like cell lines from nuclear transfer blastocysts. A green fluorescent protein (GFP)-expressing cell line was used as the source of donor cells injected into the enucleated oocytes. Blastocysts were collected at D5 (the fifth day), D7 (the seventh day) and D9 (the ninth day). Differential staining was used to assay the viability and development of blastocysts from the 3 days. The number of inner cell mass (ICM) cells increased from 1… CONTINUE READING