Isolation and culture of bovine pancreatic duct epithelial cells.

  title={Isolation and culture of bovine pancreatic duct epithelial cells.},
  author={C. Cotton and L. Al-Nakkash},
  journal={The American journal of physiology},
  volume={272 6 Pt 1},
We describe a method to isolate and culture epithelial cells from the main duct of the bovine pancreas. In primary cultures, secretin caused a dose-dependent increase in intracellular adenosine 3',5'-cyclic monophosphate (cAMP) and stimulated electrogenic transepithelial ion transport. Elevation of intracellular cAMP increased the rate coefficient for 36Cl- efflux from 0.14 +/- 0.03 to 0.47 +/- 0.12 min-1, and plasma membrane conductance, measured by the whole cell patchclamp technique, was… Expand
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