Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells
@article{Aasen2010IsolationAC, title={Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells}, author={Trond Aasen and Juan Carlos Izpisua Belmonte}, journal={Nature Protocols}, year={2010}, volume={5}, pages={371-382} }
The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In this study, we describe detailed protocols using both feeder-dependent and defined serum- and feeder-free conditions for culturing human…
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A detailed and reproducible method to derive iPSCs from plucked human hair follicle keratinocytes (HFKTs) is described, which are easily obtainable, and highly reprogrammed human cell source for all iPSC applications.
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This chapter presents a detailed a step-by-step protocol for the generation of human iPSCs from keratinocytes derived from a single plucked hair sample and discusses the future challenges in utilizing hair follicle SCs for regenerative medicine.
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