Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells

@article{Aasen2010IsolationAC,
  title={Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells},
  author={Trond Aasen and Juan Carlos Izpisua Belmonte},
  journal={Nature Protocols},
  year={2010},
  volume={5},
  pages={371-382}
}
The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In this study, we describe detailed protocols using both feeder-dependent and defined serum- and feeder-free conditions for culturing human… 
Enhanced reprogramming and cardiac differentiation of human keratinocytes derived from plucked hair follicles, using a single excisable lentivirus.
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A detailed and reproducible method to derive iPSCs from plucked human hair follicle keratinocytes (HFKTs) is described, which are easily obtainable, and highly reprogrammed human cell source for all iPSC applications.
Plucked hair: how to get stem cells and induced pluripotent stem cells for future clinical applications
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This chapter presents a detailed a step-by-step protocol for the generation of human iPSCs from keratinocytes derived from a single plucked hair sample and discusses the future challenges in utilizing hair follicle SCs for regenerative medicine.
A Comparative View on Human Somatic Cell Sources for iPSC Generation
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The advantages of keratinocytes from human plucked hair are highlighted as a widely usable, noninvasive harvesting method for primary material in comparison with other commonly used cell types.
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This chapter describes a simple and reproducible methodology for generating clinically safe nonviral, integration-free hiPSCs from keratinocytes noninvasively obtained and expanded from a donor’s single plucked hair follicle.
Isolation, cultivation and transfection of human keratinocytes
TLDR
The protocol can be used as a reproducible, simple and efficient method for isolation, cultivation and genetic manipulation of human keratinocytes, which may be useful in cell and gene therapy applications.
Induction of Pluripotent Stem Cells from Umbilical Cord Blood
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Advancing CB-derived hiPSCs towards clinical applications will require the development of completely defined conditions for hiPSC derivation and differentiation, and an evaluation of the therapeutic efficiency and safety of these cells in animal models.
Generation of human induced pluripotent stem cells from urine samples
TLDR
Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney.
Generation of Induced Pluripotent Stem Cells from Human Epidermal Keratinocytes.
TLDR
The research provides an easy and efficient method for producing iPSCs from keratinocytes, which has important applications in cell replacement therapy and demonstrates reduced DNA methylation compared with the original cells.
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