Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells

@article{Aasen2010IsolationAC,
  title={Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells},
  author={Trond Aasen and Juan Carlos Izpisua Belmonte},
  journal={Nature Protocols},
  year={2010},
  volume={5},
  pages={371-382}
}
The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In this study, we describe detailed protocols using both feeder-dependent and defined serum- and feeder-free conditions for culturing human… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 84 extracted citations

Keratinocyte Culture Techniques in Medical and Scientific Applications

Kuhn Stefanie, Radtke Christine, Allmeling Christina, M. VogtPeter, Reimers Kerstin
2017
View 4 Excerpts
Highly Influenced

Reprogramming of human hair follicle dermal papilla cells into induced pluripotent stem cells.

The Journal of investigative dermatology • 2012
View 3 Excerpts
Highly Influenced

References

Publications referenced by this paper.
Showing 1-10 of 22 references

Upping the ante: recent advances in direct reprogramming.

Molecular therapy : the journal of the American Society of Gene Therapy • 2009

Similar Papers

Loading similar papers…