Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells

Abstract

The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In this… (More)
DOI: 10.1038/nprot.2009.241

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