Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

Abstract

Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is pPy-C-G-N… (More)

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@article{Murray1976IsolationAC, title={Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.}, author={Kenneth Murray and Stephen G. Hughes and Jeffrey S. Brown and Stacey A. Bruce}, journal={The Biochemical journal}, year={1976}, volume={159 2}, pages={317-22} }