Isolation and characterization of trans-acting mutations involved in oxygen regulation of puc operon transcription in Rhodobacter sphaeroides.

@article{Lee1992IsolationAC,
  title={Isolation and characterization of trans-acting mutations involved in oxygen regulation of puc operon transcription in Rhodobacter sphaeroides.},
  author={Jeong Kug Lee and S. Kaplan},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 4},
  pages={
          1158-71
        }
}
Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-acting regulatory region involved in the expression of puc-specific mRNA, we selected Kmr colonies under aerobic conditions. Two broad classes of mutations, trans and cis, which are… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-8 of 8 citations