Isolation and characterization of three chitinases from Trichoderma harzianum.

  title={Isolation and characterization of three chitinases from Trichoderma harzianum.},
  author={Jes{\'u}s de la Cruz and A Hidalgo-Gallego and Jos{\'e} M. Lora and Tah{\'i}a Ben{\'i}tez and J. A. Pintor-Toro and Antonio Llobell},
  journal={European journal of biochemistry},
  volume={206 3},
Three proteins which display chitinase activity were purified from the supernatants of Trichoderma harzianum CECT 2413 grown in minimal medium supplemented with chitin as the sole carbon source. Purification was carried out after protein precipitation with ammonium sulphate, adsorption to colloidal chitin and digestion, and, finally, chromatofocusing. By this procedure, two chitinases of 42 kDa (CHIT42) and 37 kDa (CHIT37) were purified to homogeneity, as judged by SDS/PAGE and gel filtration… CONTINUE READING


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