Isolation and characterization of the plasma membrane biotin transporter from Schizosaccharomyces pombe.

@article{Stolz2003IsolationAC,
  title={Isolation and characterization of the plasma membrane biotin transporter from Schizosaccharomyces pombe.},
  author={Juergen Stolz},
  journal={Yeast},
  year={2003},
  volume={20 3},
  pages={
          221-31
        }
}
The fission yeast Schizosaccharomyces pombe is auxotrophic for biotin (vitamin H) and growth depends on biotin uptake over the plasma membrane. Here a biotin transport mutant of Saccharomyces cerevisiae is used to identify the vht1(+) gene encoding the Schizosaccharomyces pombe plasma membrane transport protein for biotin. SpVht1p belongs to the family of allantoate transporters and has only little sequence homology to the S. cerevisiae biotin transporter. Although having dissimilar primary… CONTINUE READING
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