Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.

@article{Fernndez1989IsolationAC,
  title={Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.},
  author={Emilio Alvarez Fern{\'a}ndez and Raymond A. Schnell and Laura P. W. Ranum and S Hussey and Carolyn D. Silflow and Paul A Lefebvre},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 17},
  pages={6449-53}
}
The nitrate reductase structural gene of Chlamydomonas reinhardtii has been isolated from a genomic library by using a nitrate reductase cDNA probe from barley. Restriction fragment length polymorphism analyses mapped the Chlamydomonas clone (B6a) to the nitrate reductase structural gene locus nit-1. Overlapping inserts cover a region of the genome of about 24 kilobases containing the entire gene, which spans approximately 5-8 kilobases. Sequence analysis of DNA fragments from the B6a clone… CONTINUE READING
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