Isolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells.

@article{Hnggi1974IsolationAC,
  title={Isolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells.},
  author={Urs J. H{\"a}nggi and John W. Littlefield},
  journal={The Journal of biological chemistry},
  year={1974},
  volume={249 5},
  pages={1390-7}
}
Dihydrofolate reductase, isolated from baby hamster kidney cells by affinity chromatography, gave multiple bands on analytical gel electrophoresis and electrofocusing. The number of bands could be reduced to two by forming a complex with the enzyme inhibitor, methotrexate. The two basic enzyme forms, called Form I and Form II, were obtained in large… CONTINUE READING