Isolation and characterization of the major protein and glycoprotein of hepatitis B surface antigen.

Abstract

Hepatitis B surface antigen has been purified and its major protein (p-25) and glycoprotein (gp-30) isolated. These isolated proteins have been subjected to amino acid analysis, Edman degradation (30 steps), carboxypeptidase digestion, and peptide mapping following tryptic hydrolysis by polyacrylamide gel electrophoresis in the presence of sodium dodecyl… (More)

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