Isolation and characterization of the main toxic fraction from the venom of the false horned viper (Pseudocerastes fieldi).

The venom of Pseudocerastes fieldi was subjected to gel filtration on Sephadex G-75. Most of the protein and lethality of the venom were eluted in a major symmetrical peak (C). The lethality of this peak is confined to a basic protein fraction, Cb (pI greater than 9.5) separable by DEAE-cellulose chromatography. Two proteins with molecular sizes close to 16… CONTINUE READING