Isolation and characterization of the gene cluster for biosynthesis of the thiopeptide antibiotic TP-1161.

@article{Engelhardt2010IsolationAC,
  title={Isolation and characterization of the gene cluster for biosynthesis of the thiopeptide antibiotic TP-1161.},
  author={Kerstin Engelhardt and Kristin F. Degnes and Sergey B Zotchev},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 21},
  pages={7093-101}
}
Recently, we isolated a new thiopeptide antibiotic, TP-1161, from the fermentation broth of a marine actinomycete typed as a member of the genus Nocardiopsis. Here we report the identification, isolation, and analysis of the TP-1161 biosynthetic gene cluster from this species. The gene cluster was identified by mining a draft genome sequence using the predicted structural peptide sequence of TP-1161. Functional assignment of a ∼16-kb genomic region revealed 13 open reading frames proposed to… CONTINUE READING