Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.

@article{Shimanuki1993IsolationAC,
  title={Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.},
  author={Mizuki Shimanuki and Noriyuki Kinoshita and Hiroyuki Ohkura and Takashi Yoshida and Takashi Toda and Mitsuhiro Yanagida},
  journal={Molecular biology of the cell},
  year={1993},
  volume={4 3},
  pages={303-13}
}
We isolated a fission yeast putative protein serine/threonine phosphatase gene designated ppe1+ by hybridization. The predicted amino acid sequence is similar to those of the fission yeast ppa2 (53% identity) and dis2 (39%) phosphatases, and highly similar to those of the budding yeast SIT4 (72%), Drosophila PPV (68%) and rabbit PPX (61%) phosphatases. Antibodies against ppe1 protein identified a 37-kd polypeptide in fission yeast. A gene disruption (designated delta ppe1) caused cold-sensitive… CONTINUE READING

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