Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.

@article{Ohtsubo1987IsolationAC,
  title={Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.},
  author={Masafumi Ohtsubo and Ren Kai and Nobuaki Furuno and Toyozo Sekiguchi and Mutsuo Sekiguchi and Hideko Hayashida and Kei-ichi Kuma and Takashi Miyata and Shinichi Fukushige and Tomoaki Murotsu},
  journal={Genes & development},
  year={1987},
  volume={1 6},
  pages={585-93}
}
The human RCC1 gene was cloned after DNA-mediated gene transfer into the tsBN2 cell line, which shows premature chromosome condensation at nonpermissive temperatures (39.5-40 degrees C). This gene codes for a 2.5-kb poly(A)+ RNA that is well conserved in hamsters and humans. We isolated 15 cDNA clones from the Okayama-Berg human cDNA library, and found two that can complement the tsBN2 mutation with an efficiency comparable to that of the genomic DNA clone. The base sequences of these two… CONTINUE READING