Isolation and characterization of the actin gene from Tetrahymena thermophila.

@article{Cupples1986IsolationAC,
  title={Isolation and characterization of the actin gene from Tetrahymena thermophila.},
  author={Claire G. Cupples and Ronald E. Pearlman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 14},
  pages={5160-4}
}
The macronucleus of Tetrahymena thermophila contains a single actin gene. We have isolated this gene from a partial plasmid library by using the yeast actin gene as a probe. The nucleotide sequence of the gene has been determined and the amino acid sequence of the potential protein deduced. The encoded protein is 375 amino acids long, one amino acid longer than the yeast actin. It is one of the most divergent actins sequenced to date, being only 75% homologous to yeast actin. Unlike the actin… CONTINUE READING