Isolation and characterization of the 54-kDa and 22-kDa chitinase genes of Serratia marcescens KCTC2172.

@article{Gal1997IsolationAC,
  title={Isolation and characterization of the 54-kDa and 22-kDa chitinase genes of Serratia marcescens KCTC2172.},
  author={Sang Wan Gal and J Y Choi and Chang Yub Kim and Yong Hwa Cheong and Young Jun Choi and Jeong Dong Bahk and Si Young Lee and Moo Je Cho},
  journal={FEMS microbiology letters},
  year={1997},
  volume={151 2},
  pages={197-204}
}
A DNA fragment (pCHI5422) containing two genes encoding a 54-kD and a 22-kDa chitinase was isolated from a cosmid DNA library of Serratia marcescens KCTC2172. The complete nucleotide sequence of pCHI5422 consisting of 4581 bp was determined. The nucleotide sequence of the 22-kDa chitinase consists of 681 bp of open reading frame encoding 227 amino acids and is located 1422 bp downstream of the translation termination codon of the 54-kDa chitinase sequence. The 54-kDa chitinase gene consisted of… CONTINUE READING
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