Isolation and characterization of sulfur globule proteins from Chromatium vinosum and Thiocapsa roseopersicina

  title={Isolation and characterization of sulfur globule proteins from Chromatium vinosum and Thiocapsa roseopersicina},
  author={D. Brune},
  journal={Archives of Microbiology},
  • D. Brune
  • Published 2004
  • Chemistry, Medicine
  • Archives of Microbiology
  • Purple sulfur bacteria store sulfur as intracellular globules enclosed by a protein envelope. The proteins associated with sulfur globules of Chromatium vinosum and Thiocapsa roseopersicina were isolated by extraction into 50% aqueous acetonitrile containing 1% trifluoroacetic acid and 10 mM dithiothreitol. The extracted proteins were separated by reversed-phase HPLC, revealing three major proteins from C. vinosum and two from T. roseopersicina. All of these proteins have similar, rather… CONTINUE READING
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