Isolation and characterization of spinach photosystem II membrane-associated catalase and polyphenol oxidase.

@article{Sheptovitsky1996IsolationAC,
  title={Isolation and characterization of spinach photosystem II membrane-associated catalase and polyphenol oxidase.},
  author={Y G Sheptovitsky and Gary W Brudvig},
  journal={Biochemistry},
  year={1996},
  volume={35 50},
  pages={
          16255-63
        }
}
Photosystem II (PSII) membranes exhibit catalase and polyphenol oxidase (PPO) activities. Mild heat treatment of PSII membranes for 90 min at 30 degrees C releases most of these enzyme activities into the supernatant, accompanied by a 7-fold activation of PPO. In contrast, mild heat treatment of thylakoid membranes does not release significant amounts of either activity, indicating that both enzymes are bound to the luminal surface of the thylakoid membrane. The heat-released PSII membrane… CONTINUE READING
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