Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes.

Abstract

The moderate halophile Halomonas elongata Deustche Sammlung für Mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 M NaCl). Two Tn1732-induced mutants, CHR62 and CHR63, that were severely affected in their salt tolerance were isolated. Mutant CHR62 could not grow above 0.75 M NaCl, and CHR63… (More)

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@article{Cnovas1997IsolationAC, title={Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes.}, author={David C{\'a}novas and Carmen Vargas and Fernando Iglesias-Guerra and L{\'a}szl{\'o} N. Csonka and David Rhodes and Ant{\'o}nio Ventosa and Joaqu{\'i}n J. Nieto}, journal={The Journal of biological chemistry}, year={1997}, volume={272 41}, pages={25794-801} }