Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.

@article{Sia2001IsolationAC,
  title={Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.},
  author={Elaine A Sia and Margaret Dominska and L. V. Stefanovi{\'c} and Thomas D Petes},
  journal={Molecular and cellular biology},
  year={2001},
  volume={21 23},
  pages={8157-67}
}
The stability of simple repetitive DNA sequences (microsatellites) is a sensitive indicator of the ability of a cell to repair DNA mismatches. In a genetic screen for yeast mutants with elevated microsatellite instability, we identified strains containing point mutations in the yeast mismatch repair genes, MSH2, MSH3, MLH1, and PMS1. Some of these mutations conferred phenotypes significantly different from those of null mutations in these genes. One semidominant MSH2 mutation was identified… CONTINUE READING