Isolation and characterization of ornithine transcarbamylase from normal human liver.

@article{Kalousek1978IsolationAC,
  title={Isolation and characterization of ornithine transcarbamylase from normal human liver.},
  author={Frantisek Kalousek and Baudouin François and Leon E. Rosenberg},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 11},
  pages={3939-44}
}
We report experiments describing the isolation and characterization of ornithine transcarbamylase from normal human liver. Our preparative procedure employs initial centrifugation and heat steps, intermediate batch-wise adsorption and desorption from ion exchange resins and column chromatographic elution from hydroxylapatite, and final purification by gel filtration chromatography and glycerol density gradient centrifugation. The enzyme, purified 580-fold in this way, is homogeneous as judged… CONTINUE READING
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