Isolation and characterization of neutral-lipid-containing organelles and globuli-filled plastids from Brassica napus tapetum.

@article{Wu1997IsolationAC,
  title={Isolation and characterization of neutral-lipid-containing organelles and globuli-filled plastids from Brassica napus tapetum.},
  author={Sherry S. H. Wu and Kathryn A. Platt and Chandra Ratnayake and T W Wang and Julie T. L. Ting and Anthony H C Huang},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 23},
  pages={12711-6}
}
The monolayer tapetum cells of the maturing flowers of Brassica napus contain abundant subcellular globuli-filled plastids and special lipid particles, both enriched with lipids that are supposed to be discharged and deposited onto the surface of adjacent maturing pollen. We separated the two organelles by flotation density gradient centrifugation and identified them by electron microscopy. The globuli-filled plastids had a morphology similar to those described in other plant species and… CONTINUE READING
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