Isolation and characterization of nucleases from a clinical isolate of Serratia marcescens kums 3958.

@article{Yonemura1983IsolationAC,
  title={Isolation and characterization of nucleases from a clinical isolate of Serratia marcescens kums 3958.},
  author={Kimie Yonemura and Kazuhiko Matsumoto and Hideaki Maeda},
  journal={Journal of biochemistry},
  year={1983},
  volume={93 5},
  pages={
          1287-95
        }
}
Two new extracellular nucleases, nucleases SM1 and SM2, were purified from the culture fluid of S. marcescens kums 3958, a fresh clinical isolate. The purification was carried out by the following steps; ammonium sulfate precipitation, and DEAE-cellulose and Sephadex G-100 column chromatography. At the final step, nucleases SM1 and SM2 were purified about 3,700- and 1,000-fold, respectively. They were free from phosphomonoesterase and phosphodiesterase activities. The pIs were 8.1 and 7.5 for… CONTINUE READING