Isolation and characterization of new strains of methanogens from cold terrestrial habitats.

@article{Simankova2003IsolationAC,
  title={Isolation and characterization of new strains of methanogens from cold terrestrial habitats.},
  author={Maria V Simankova and Oleg Kotsyurbenko and Tillmann Lueders and Alla N. Nozhevnikova and Bianca Wagner and Ralf Conrad and Michael W. Friedrich},
  journal={Systematic and applied microbiology},
  year={2003},
  volume={26 2},
  pages={
          312-8
        }
}
Five strains of methanogenic archaea (MT, MS, MM, MSP, ZB) were isolated from permanently and periodically cold terrestrial habitats. Physiological and morphological studies, as well as phylogenetic analyses of the new isolates were performed. Based on sequences of the 16S rRNA and methyl-coenzyme M reductase a-subunit (mcrA) genes all new isolates are closely related to known mesophilic and psychrotolerant methanogens. Both, phylogenetic analyses and phenotypic properties allow to classify… 
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References

SHOWING 1-10 OF 26 REFERENCES

Methanosarcina lacustris sp. nov., a new psychrotolerant methanogenic archaeon from anoxic lake sediments.

Based on the results of physiological and phylogenetic studies strain ZS can be assigned to a new species of the genus Methanasarcina, the name Methanosarcina lacustris sp.

A Methanogenic Archaeon from Ace Lake, Antarctica: Methanococcoides burtonii sp. nov.

Isolation and Characterization of Methanomethylovorans hollandica gen. nov., sp. nov., Isolated from Freshwater Sediment, a Methylotrophic Methanogen Able To Grow on Dimethyl Sulfide and Methanethiol

It is proposed that strain DMS1T is a representative of a novel genus which was directly enriched and isolated from a freshwater sediment in defined minimal medium containing dimethyl sulfide as the sole carbon and energy source and was named Methanomethylovorans hollandica.

Preliminary observations on psychrotrophic and psychrophilic, heterotrophic bacteria from antarctic water samples

Identification of 93 isolates showed the majority to be Pseudomonas (82%), with Flavobacterium and Moraxella representing 13% of the isolates, and this strain may represent a new taxon of curved, gram negative bacteria.

Diversity and Taxonomy of Methanogens

This work has shown that methane production is a ubiquitous, defining characteristic of methanogens, a group of microbes that is phylogenetically distinct from eukaryotes and true bacteria.

Examination of Antarctic prokaryotic diversity through molecular comparisons

  • P. Franzmann
  • Biology, Environmental Science
    Biodiversity & Conservation
  • 2004
Use of the 16S rRNA gene as a molecular clock would suggest that the majority of Antarctic prokaryotes diverged from their nearest known non-Antarctic relatives long before a stable ice-sheet developed in Antarctica.

Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica.

This is the first report of a psychrophilic methanogen growing by CO2 reduction, isolated from the perennially cold, anoxic hypolimnion of Ace Lake in the Vesfold Hills of Antarctica.

Methanosarcina semesiae sp. nov., a dimethylsulfide-utilizing methanogen from mangrove sediment.

Analysis of the 16S rRNA gene sequence showed that strain MD1T was phylogenetically closely related to members of the genus Methanosarcina, but clearly differed from all described species of this genus.

Methanogenesis at low temperatures by microflora of tundra wetland soil

Kinetic analysis of the results confirmed the prevalence of hydrogen acetogenesis on methanogenesis, and accumulation of acetate- and H2/CO2-utilising methanogens should be very important in methanogenic microbial community operating at low temperatures.

Methanogenesis in sediments from deep lakes at different temperatures (2–70°C)

The communities enriched at 4–8°C had the highest activity at low temperatures indicating that a specific psychrophilic community exists, and methane production from organic matter of sediments occurred at all temperatures tested.