Isolation and characterization of new strains of methanogens from cold terrestrial habitats.

@article{Simankova2003IsolationAC,
  title={Isolation and characterization of new strains of methanogens from cold terrestrial habitats.},
  author={Maria V Simankova and Oleg Rollandovich Kotsyurbenko and Tillmann Lueders and Alla N. Nozhevnikova and Bianca Wagner and Ralf Conrad and Michael W. Friedrich},
  journal={Systematic and applied microbiology},
  year={2003},
  volume={26 2},
  pages={
          312-8
        }
}
Five strains of methanogenic archaea (MT, MS, MM, MSP, ZB) were isolated from permanently and periodically cold terrestrial habitats. Physiological and morphological studies, as well as phylogenetic analyses of the new isolates were performed. Based on sequences of the 16S rRNA and methyl-coenzyme M reductase a-subunit (mcrA) genes all new isolates are closely related to known mesophilic and psychrotolerant methanogens. Both, phylogenetic analyses and phenotypic properties allow to classify… Expand
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References

SHOWING 1-10 OF 26 REFERENCES
Methanosarcina lacustris sp. nov., a new psychrotolerant methanogenic archaeon from anoxic lake sediments.
TLDR
Based on the results of physiological and phylogenetic studies strain ZS can be assigned to a new species of the genus Methanasarcina, the name Methanosarcina lacustris sp. Expand
A Methanogenic Archaeon from Ace Lake, Antarctica: Methanococcoides burtonii sp. nov.
TLDR
A methylotrophic, methanogenic bacterium was isolated from the anoxic hypolimnion of Ace Lake, Antarctica, a lake with ionic composition similar to that of seawater, where a new species Methanococcoides burtonii is described and the type strain is DSM 6242. Expand
Isolation and Characterization of Methanomethylovorans hollandica gen. nov., sp. nov., Isolated from Freshwater Sediment, a Methylotrophic Methanogen Able To Grow on Dimethyl Sulfide and Methanethiol
TLDR
It is proposed that strain DMS1T is a representative of a novel genus which was directly enriched and isolated from a freshwater sediment in defined minimal medium containing dimethyl sulfide as the sole carbon and energy source and was named Methanomethylovorans hollandica. Expand
Preliminary observations on psychrotrophic and psychrophilic, heterotrophic bacteria from antarctic water samples
TLDR
Identification of 93 isolates showed the majority to be Pseudomonas (82%), with Flavobacterium and Moraxella representing 13% of the isolates, and this strain may represent a new taxon of curved, gram negative bacteria. Expand
Diversity and Taxonomy of Methanogens
TLDR
This work has shown that methane production is a ubiquitous, defining characteristic of methanogens, a group of microbes that is phylogenetically distinct from eukaryotes and true bacteria. Expand
Molecular analyses of methyl-coenzyme M reductase alpha-subunit (mcrA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage.
TLDR
A new methanogen-specific fingerprinting assay based on terminal restriction fragment length polymorphism (T-RFLP) analysis of fluorescently labelled polymerase chain reaction (PCR) products allowed us to distinguish all environmental mcrA/mrtA sequences via group-specific Sau96I restriction sites. Expand
Examination of Antarctic prokaryotic diversity through molecular comparisons
TLDR
Use of the 16S rRNA gene as a molecular clock would suggest that the majority of Antarctic prokaryotes diverged from their nearest known non-Antarctic relatives long before a stable ice-sheet developed in Antarctica. Expand
Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica.
TLDR
This is the first report of a psychrophilic methanogen growing by CO2 reduction, isolated from the perennially cold, anoxic hypolimnion of Ace Lake in the Vesfold Hills of Antarctica. Expand
Methanosarcina semesiae sp. nov., a dimethylsulfide-utilizing methanogen from mangrove sediment.
TLDR
Analysis of the 16S rRNA gene sequence showed that strain MD1T was phylogenetically closely related to members of the genus Methanosarcina, but clearly differed from all described species of this genus. Expand
Methanogenesis at low temperatures by microflora of tundra wetland soil
TLDR
Kinetic analysis of the results confirmed the prevalence of hydrogen acetogenesis on methanogenesis, and accumulation of acetate- and H2/CO2-utilising methanogens should be very important in methanogenic microbial community operating at low temperatures. Expand
...
1
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3
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