Isolation and characterization of new strains of methanogens from cold terrestrial habitats.

  title={Isolation and characterization of new strains of methanogens from cold terrestrial habitats.},
  author={Maria V Simankova and Oleg Kotsyurbenko and Tillmann Lueders and Alla N. Nozhevnikova and Bianca Wagner and Ralf Conrad and Michael W. Friedrich},
  journal={Systematic and applied microbiology},
  volume={26 2},
Five strains of methanogenic archaea (MT, MS, MM, MSP, ZB) were isolated from permanently and periodically cold terrestrial habitats. Physiological and morphological studies, as well as phylogenetic analyses of the new isolates were performed. Based on sequences of the 16S rRNA and methyl-coenzyme M reductase a-subunit (mcrA) genes all new isolates are closely related to known mesophilic and psychrotolerant methanogens. Both, phylogenetic analyses and phenotypic properties allow to classify… 

Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark).

Based on 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel taxon within the genus Methanobacterium, namely MethanOBacterium aarhusense sp.

Aceticlastic and NaCl-Requiring Methanogen “Methanosaeta pelagica” sp. nov., Isolated from Marine Tidal Flat Sediment

The number of Methanosaeta organisms increased with increasing depth and was much higher than that of Methanosarcina organisms, suggesting that aceticlastic methanogens contribute to acetate metabolism to a greater extent than previously thought in marine environments, where sulfate-reducing acetate oxidation prevails.

Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments.

  • K. MoriS. Harayama
  • Biology, Engineering
    International journal of systematic and evolutionary microbiology
  • 2011
Two methane-producing archaea, designated Mic5c12(T) and Mic6c05(T), were isolated from sludge deposited in a crude oil storage tank and a tubercle on the interior of a pipe transporting natural gas-containing brine, respectively and revealed that they belonged to the genus Methanobacterium.

Methanobacterium aggregans sp. nov., a hydrogenotrophic methanogenic archaeon isolated from an anaerobic digester.

A novel, strictly anaerobic, hydrogenotrophic methanogen, strain E09F.3T, which was isolated from a commercial biogas plant in Germany and was closely related to Methanobacterium congolense CT but morphological, physiological and genomic characteristics indicated that it represents a novel species.

Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern

The first draft metagenome-assembled genome (MAG) sequence of an unclassified Methanosarcinaceae methanogen phylogenetically placed adjacent to the Methanolobus and Methanomethylovorans genera that appears to be a distinct genus and species is presented.

Methanospirillum lacunae sp. nov., a methane-producing archaeon isolated from a puddly soil, and emended descriptions of the genus Methanospirillum and Methanospirillum hungatei.

On the basis of its phenotypic characteristics and phylogenetic position, strain Ki8-1(T) is considered to represent a novel species of the genus Methanospirillum, for which the name Methan Hospicellum lacunae sp.

Archaeal communities of Arctic methane-containing permafrost.

Investigation of the diversity of methanogenic archaea and their distribution in five permafrost samples collected from a borehole in the Kolyma River Lowland found the greatest diversity of archaea was observed at a depth of 22.3 m, probably due to the availability of the labile organic carbon and/or to the migration of the microbial cells during the freezing front towards the bottom.

Isolation of Uncultivated Methanogens using Anaerobic Syntrophic Substrate-degrading Coculture System.

Results indicate that an anaerobic syntrophic substrate-degrading coculture system could be used as an effective method of isolating uncultivated methanogens.

Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.

Phenotypic, physiological and genomic characteristics indicated that strain E03.2T represents a novel species of the genus Methanosarcina, for which the name MethanosARCina flavescens sp.

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

Phylogenetic analysis of 16S rRNA and mcrA genes demonstrated that strains KOR-3, -4, -5, and -6 are related to Methanosarcina mazei (M. mazei, 99% sequence similarity); however, chloramphenicol inhibited cell growth.



Methanosarcina lacustris sp. nov., a new psychrotolerant methanogenic archaeon from anoxic lake sediments.

Based on the results of physiological and phylogenetic studies strain ZS can be assigned to a new species of the genus Methanasarcina, the name Methanosarcina lacustris sp.

Isolation and Characterization of Methanomethylovorans hollandica gen. nov., sp. nov., Isolated from Freshwater Sediment, a Methylotrophic Methanogen Able To Grow on Dimethyl Sulfide and Methanethiol

It is proposed that strain DMS1T is a representative of a novel genus which was directly enriched and isolated from a freshwater sediment in defined minimal medium containing dimethyl sulfide as the sole carbon and energy source and was named Methanomethylovorans hollandica.

Preliminary observations on psychrotrophic and psychrophilic, heterotrophic bacteria from antarctic water samples

Identification of 93 isolates showed the majority to be Pseudomonas (82%), with Flavobacterium and Moraxella representing 13% of the isolates, and this strain may represent a new taxon of curved, gram negative bacteria.

Diversity and Taxonomy of Methanogens

This work has shown that methane production is a ubiquitous, defining characteristic of methanogens, a group of microbes that is phylogenetically distinct from eukaryotes and true bacteria.

Examination of Antarctic prokaryotic diversity through molecular comparisons

  • P. Franzmann
  • Biology, Environmental Science
    Biodiversity & Conservation
  • 2004
Use of the 16S rRNA gene as a molecular clock would suggest that the majority of Antarctic prokaryotes diverged from their nearest known non-Antarctic relatives long before a stable ice-sheet developed in Antarctica.

Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica.

This is the first report of a psychrophilic methanogen growing by CO2 reduction, isolated from the perennially cold, anoxic hypolimnion of Ace Lake in the Vesfold Hills of Antarctica.

Methanosarcina semesiae sp. nov., a dimethylsulfide-utilizing methanogen from mangrove sediment.

Analysis of the 16S rRNA gene sequence showed that strain MD1T was phylogenetically closely related to members of the genus Methanosarcina, but clearly differed from all described species of this genus.

Methanogenesis at low temperatures by microflora of tundra wetland soil

Kinetic analysis of the results confirmed the prevalence of hydrogen acetogenesis on methanogenesis, and accumulation of acetate- and H2/CO2-utilising methanogens should be very important in methanogenic microbial community operating at low temperatures.

Methanogenesis in sediments from deep lakes at different temperatures (2–70°C)

The communities enriched at 4–8°C had the highest activity at low temperatures indicating that a specific psychrophilic community exists, and methane production from organic matter of sediments occurred at all temperatures tested.