Isolation and characterization of mutations affecting expression of the delta9- fatty acid desaturase gene, OLE1, in Saccharomyces cerevisiae.

@article{Fujimori1997IsolationAC,
  title={Isolation and characterization of mutations affecting expression of the delta9- fatty acid desaturase gene, OLE1, in Saccharomyces cerevisiae.},
  author={Ko Fujimori and Sarintip Anamnart and Yasuhito Nakagawa and Shigemi Sugioka and Daisaku Ohta and Yasuji Oshima and Yasuhiro Yamada and S. Harashima},
  journal={FEBS letters},
  year={1997},
  volume={413 2},
  pages={226-30}
}
Expression of the delta9- fatty acid desaturase gene, OLE1, of Saccharomyces cerevisiae is negatively regulated transcriptionally and post-transcriptionally by unsaturated fatty acids. In order to isolate mutants exhibiting irregulation of OLE1 expression, we constructed an OLE1p-PHO5 fusion gene as a reporter consisting of the PHO5 gene encoding repressible acid phosphatase (rAPase) under the control of the OLE1 promoter (OLE1p). By EMS mutagenesis, we isolated three classes of mutants, pfo1… CONTINUE READING