Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae

@article{Tsukada1993IsolationAC,
  title={Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae},
  author={Miki Tsukada and Yoshinori Ohsumi},
  journal={FEBS Letters},
  year={1993},
  volume={333}
}
AUT 1 , a Gene Essential for Autophagocytosis in the Yeast Saccharomyces cerevisiae
TLDR
Deletion of the AUT1 gene had no obvious influence on secretion, fluid phase endocytosis, or vacuolar protein sorting, which supports the idea of autophagocytotic as being a novel route transporting proteins from the cytoplasm to the vacuole.
Starvation Induced Cell Death in Autophagy-Defective Yeast Mutants Is Caused by Mitochondria Dysfunction
TLDR
Under starvation conditions non-selective autophagy, rather than mitophagy plays an essential role in preventing ROS accumulation, and thus in maintaining mitochondria function, and the failure of response to starvation is the major cause of cell death in atg mutants.
AUT1, a gene essential for autophagocytosis in the yeast Saccharomyces cerevisiae
TLDR
Deletion of the AUT1 gene had no obvious influence on secretion, fluid phase endocytosis, or vacuolar protein sorting, which supports the idea of autophagocytotic as being a novel route transporting proteins from the cytoplasm to the vacuole.
Autophagy and the cvt Pathway Both Depend onAUT9
TLDR
The AUT9 gene is identified and characterized, which is allelic with CVT7.
Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation.
TLDR
It is shown that processing of GFP-tagged Atg8 can serve as a marker for autophagy in the fission yeast Schizosaccharomyces pombe, and evidence for the existence of a recycling system for nitrogen sources during starvation is sought.
An ARL1 mutation affected autophagic cell death in yeast, causing a defect in central vacuole formation
TLDR
The ARL1 gene plays an important role in the formation of central vacuoles and in the progression of programmed cell death induced by cell-cycle arrest or Bax, suggesting the presence of a programmed-cell death machinery in yeast that is similar to that related to the Type II cell death of mammalian cells characterized by autophagocytosis.
AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae
Autophagocytosis is a starvation-induced process, carrying proteins destined for degradation to the lysosome. In the yeast Saccharomyces cerevisiae, the autophagic process is visualized by the
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