Isolation and characterization of muscle membranes using surface-specific labels.

@article{Barchi1977IsolationAC,
  title={Isolation and characterization of muscle membranes using surface-specific labels.},
  author={Robert L. Barchi and Eduardo Bonilla and M Wong},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1977},
  volume={74 1},
  pages={
          34-8
        }
}
Membranes were purified from rat muscle by a differential centrifugation method that avoids the use of salt extraction or incubations at elevated temperature. Three populations of membrane-limited vesicles were defined having average densities of 1.112 (fraction I), 1.141 (fraction II), and 1.158 (fraction III) g/ml in a continuous sucrose gradient. Lactoperoxidase-catalyzed iodination of intact muscle prior to isolation of membranes resulted in highest specific activity in fraction I, although… CONTINUE READING