Isolation and characterization of microsatellites in Brassica rapa L.

@article{Suwabe2002IsolationAC,
  title={Isolation and characterization of microsatellites in Brassica rapa L.},
  author={Keita Suwabe and Hiroyuki Iketani and Tsukasa Nunome and Takahiro Kage and Masashi Hirai},
  journal={Theoretical and Applied Genetics},
  year={2002},
  volume={104},
  pages={1092-1098}
}
Abstract.We report here the isolation and characterization of microsatellites, or simple sequence repeats (SSRs), in Brassica rapa. The size-fractionated genomic library was screened with (GA)15 and (GT)15 oligonucleotide probes. A total of 58 clones were identified as having the microsatellite repeats, and specific primer pairs were designed for 38 microsatellite loci. All primer pairs, except two, amplified fragments having the sizes expected from the sequences. Of the 36 primer pairs, 35… 
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Genic Microsatellite Markers in Brassica rapa: Development, Characterization, Mapping, and Their Utility in Other Cultivated and Wild Brassica Relatives
  • N. Ramchiary, Van Dan Nguyen, +6 authors Y. Lim
  • Biology, Medicine
    DNA research : an international journal for rapid publication of reports on genes and genomes
  • 2011
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A novel locus for clubroot resistance in Brassica rapa and its linkage markers
TLDR
The present study clearly demonstrates that B. rapa has at least three CR loci, and the new CR locus was named Crr3, which may be useful in breeding CR Chinese cabbage cultivars to overcome the decay of present CR cultivars.
Development of microsatellite markers by ISSR-suppression-PCR method in Brassica rapa
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The development and sharing of microsatellite markers which involve a comparison of different linkage maps, thus facilitate future marker-assisted selection for the breeding of oilseed and vegetable crops and the ISSR-suppression-PCR method is proposed.
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