Isolation and characterization of microsatellite markers for an important tropical tree, Aquilaria malaccensis (Thymelaeaceae).

@article{Tnah2012IsolationAC,
  title={Isolation and characterization of microsatellite markers for an important tropical tree, Aquilaria malaccensis (Thymelaeaceae).},
  author={Lee Hong Tnah and Chai Ting Lee and Soon Leong Lee and Kevin Kit Siong Ng and Chin Hong Ng and Zakaria Nurul-Farhanah and Kah Hoo Lau and Lillian Swee Lian Chua},
  journal={American journal of botany},
  year={2012},
  volume={99 11},
  pages={
          e431-3
        }
}
PREMISE OF THE STUDY Aggressive collections and trade activities in recent decades have resulted in heavy pressure on the natural stands of Aquilaria malaccensis and concerns over its long-term survival potential. To aid DNA profiling and assessment of its genetic diversity, microsatellite markers were developed for the species. METHODS AND RESULTS Seventeen polymorphic microsatellite markers were developed for A. malaccensis using an enrichment protocol. The markers were screened on 24… 
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11 Citations

Isolation and characterization of 16 microsatellite markers in Intsia palembanica, a high-value tropical hardwood species
TLDR
These microsatellite markers were developed for Intsia palembanica using an enrichment approach and will be useful for conservation genetic study, individual identification and timber tracking.
Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes.
TLDR
All markers described in this study will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of Bixa orellana.
Analysis of genetic variability in Aquilaria malaccensis from Bramhaputra valley, Assam, India using ISSR markers
Molecular identification of Aquilaria spp. by using inter-simple sequence repeat (ISSR)
TLDR
Inter-simple sequence repeat (ISSR) markers are highly polymorphic and have high reproducibility which will be useful in areas of genetic diversity, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of crop species.
Cross-amplification of microsatellite markers across agarwood-producing species of the Aquilarieae tribe (Thymelaeaceae)
TLDR
It is demonstrated that genetic fingerprinting can be an effective tool in helping to manage agarwood genetic resources by potentially supporting the chain-of-custody of agar wood and its products in the market.
Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
TLDR
The pattern of genetic variation among wild populations was measured to aid in designing useful strategies for in situ conservation and implies that geographical segregation is a contributing factor toward genetic variation in A. malaccensis.
DNA databases of a CITES listed species Aquilaria malaccensis (Thymelaeaceae) as the tracking tools for forensic identification and chain of custody certification.
Towards conservation strategies for forest tree endangered species: the meaning of population genetic statistics
TLDR
How estimates such as mean number of alleles, heterozigosity, fixation index, outcrossing rate, gene flow, and genetic differentiation can assist in the development of a conservation program for tree species are reviewed and discussed.
Development of protocols for genomic library construction of Agarwood (Aquilaria malaccensis)
TLDR
This research aimed at establishing cloning protocols for genomic library construction of agarwood tree (Aquilaria malaccensis) until sequencing process, indicating that the genomic region in this library construction is different from the genomic DNA sequences of A.malaccensis in NCBI.
AGARWOOD BIBLIOGRAPHY A COMPILATION OF ABSTRACTS ON AGARWOOD STUDIES
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References

SHOWING 1-5 OF 5 REFERENCES
Conservation and sustainable use of tropical trees in the genus Aquilaria I. Status and distribution in Indonesia.
Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles.
TLDR
A method is described for the rapid isolation of microsatellite sequences using a biotin-labeled oligonucleotide attached to streptavidin-coated magnetic particles that is applicable to any genome and in principle is adaptable to the rapid isolated of both repetitive as well as genic sequences.
Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment
TLDR
It is shown that the likelihood equations used by versions 1.0 and 2.0 of cervus to accommodate genotyping error miscalculate the probability of observing an erroneous genotype, and that correcting this error increases success in paternity assignment.
Rapid isolation of high molecular weight plant DNA.
TLDR
A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA.
Aquilaria malaccensis Lam