Isolation and characterization of microsatellite markers from the great hornbill, Buceros bicornis

  title={Isolation and characterization of microsatellite markers from the great hornbill, Buceros bicornis},
  author={Siriphatr Chamutpong and D. Saito and Nareerat Viseshakul and Isao Nishiumi and Pilai Poonswad and Mathurose Ponglikitmongkol},
  journal={Molecular Ecology Resources},
Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean polymorphic information content was 0.62. Among these, three loci deviated from the Hardy–Weinberg equilibrium. However, no significant genotypic disequilibrium was detected… 

Fourteen new microsatellite markers for the Visayan tarictic hornbill (Penelopides panini) and their cross-species applicability among other endangered Philippine hornbills

These 14 new microsatellite markers specifically developed for two genera of Philippine hornbills, in combination with those already available for the hornbill genera Buceros and Bucorvus, comprise a reasonable number of loci to genetically analyse wild and captive populations of these and probably other related, often endangered hornbilling species.

Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss

Mitochondrial and nuclear data concordantly show a clear genetic separation according to the island of origin in both Penelopides species, but also unravel sporadic over-water movements between islands, suggesting deforestation in the last century influenced these migratory events.


This report is the fi rst report of hybridisation between the great hornbill and the rhinoceros hornbill in the wild, and has been genetically confi rmed.

The complete mitochondrial genome sequence of Buceros bicornis (Bucerotiformes: Bucerotidae)

The newly obtained Mitogenome sequence seems to be useful for conservation biology and evolutionary studies on hornbill bird species, which would contribute to enrich the Buceros bicornis mitogenome resource and promote its biological research.



Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites.

A blunt-end linker, named SNX for its restriction sites, that allows the use of combinations of restriction enzymes to digest the majority of genomic DNA into the 200-1000-bp range, resulting in representative small-insert libraries with a very high proportion of positive clones.

Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping.

It was clear, however, that features of the template in addition to the 3' terminal base also affect the fraction of product adenylated, and it proved difficult to identify a single sequence capable of protecting the products of all markers from non-templated addition of nucleotide.

Primer3 on the WWW for general users and for biologist programmers.

This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs.

Statistical confidence for likelihood‐based paternity inference in natural populations

This study derives likelihood ratios for paternity inference with codominant markers taking account of typing error, and defines a statistic Δ for resolving paternity, and demonstrates the method is robust to their presence under commonly encountered conditions.

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Current status and distribution of hornbills and their habitat in Thailand

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