Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character

@article{Gunge1981IsolationAC,
  title={Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character},
  author={Norio Gunge and Atsuko Tamaru and Fujiko Ozawa and Kenji Sakaguchi},
  journal={Journal of Bacteriology},
  year={1981},
  volume={145},
  pages={382 - 390}
}
Two linear deoxyribonucleic acid plasmids, designated pGK11 and pGK12, were isolated from the yeast Kluyveromyces lactis IFO 1267. pGK11 and pGK12 had molecular weights of 5.4 X 10(6) and 8.4 X 10(6), respectively. Both plasmids possessed the same density of 1.687 g/cm3, lighter than the densities of mitochondrial (1.692 g/cm3) and nuclear (1.699 g/cm3) deoxyribonucleic acids. A restriction map of pGK11 was constructed from digestions by EcoRI, HindIII, PstI, and BamHI. pGK12 was cleaved by… 
Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion
TLDR
Both the killer and the resistance genes are located on the pGKl plasmids, which replicated autonomously and stably in the new host cells of S. cerevisiae and could coexist with the resident 2-micrometers deoxyribonucleic acid plasmid.
Killer DNA Plasmids of the Yeast Kluyveromyces lactis
TLDR
The plasmid DNA of K. lactis showed no detectable sequence homology with the double stranded RNA of the killer system of Saccharomyces cerevisiae and neither was any homology found with nuclear and mitochondria) DNA.
Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis.
TLDR
Lactis toxin was effective with sensitive strains of S. cerevisiae in a relatively wider pH range between 4 and 8; it was stable for several months at pH 6.0 when stored below -20 degrees C.
Linear DNA plasmids from Pichia etchellsii, Debaryomyces hansenii and Wingea robertsiae.
TLDR
Linear DNA plasmids were found in the following yeasts: four strains of Kluyveromyces lactis, one of Debaryomyces hansenii, two of Wingea robertsiae and four of Pichia etchellsii, suggesting related genome organization of these plasmid genes.
The linear plasmids of Kluyveromyces lactis: Genetic and molecular analysis.
THE LINEAR PLASMIDS OF KLUYVEROMYCES LACTIS GENETIC AND MOLECULAR ANALYSIS Duncan W.Wilson Strains of the budding-yeast Kluyveromyces 2 act is that produce killer-toxin contain two linear dsDNA
Structure of a linear plasmid of the yeast Kluyveromyces lactis; Compact organization of the killer genome
TLDR
The total nucleotide sequence of k1 DNA, a pair of linear DNA plasmids, is determined and it is shown that these genes are transcribed from one strand and the complementary strand and has terminal inverted repeats of 202 base pairs.
Replication and maintenance of the Kluyveromyces linear pGKL plasmids
TLDR
It was concluded that the reason for the pGKL1 dependent replication of the pK192L/S plasmids was the absence of the intact pG kL1-ORF1 gene and that the ORF1 function was necessary for the replication ofThe pGkL1 genome.
Linear DNA plasmids of Pichia inositovora are associated with a novel killer toxin activity
TLDR
Pichia inositovora strains cured of their linear plasmids do not become detectably sensitive to toxin produced by the wild-type strain, suggesting a nonplasmid-encoded immunity function.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 33 REFERENCES
A LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP. PRODUCING LANKACIDIN GROUP ANTIBIOTICS
TLDR
Results suggest that the linear plasmid-like DNA was isolated from a lysate of Streptomyces sp.
Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase.
TLDR
The Escherichia coli R factor-derived chloramphenicol resistance (camr) gene is functionally expressed in the yeast Saccharomyces cerevisiae and segregate with the yeast marker LEU2, carried by the transforming plasmid, during both mitotic growth and meiotic division.
GENETIC ANALYSIS OF PRODUCTS OF PROTOPLAST FUSION IN SACCHAROMYCES CEREVISIAE
TLDR
It was concluded that the protoplast fusion proceeded to karyogamy to produce stable diploids, suggesting that the fusion products were a/a/α/α tetraploids.
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
TLDR
A set of vector DNAs useful for the cloning of DNA fragments in Saccharomyces cerevisiae (yeast) and in Escherichia coli are characterized and general methods for the selection and the analysis of these events are presented.
Closed-Circular DNA from Mitochondrial-Enriched Fractions of Four Petite-Negative Yeasts
TLDR
The finding of a gross size difference between mitochondrial DNA from petite-negative yeasts and Saccharomyces cerevisiae focuses attention on the suggestion that certain unique properties of the latter organism's mitochodrial DNA may be of relevance to the mechanism of the petite mutation in yeast.
...
1
2
3
4
...