Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium.

@article{Callen1994IsolationAC,
  title={Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium.},
  author={A M Callen and Andr{\'e} Adoutte and J. Mason Andrew and Anne Baroin-Tourancheau and M. H. Br{\'e} and Phil Ruiz and J C Cl{\'e}rot and Patricia Delgado and Anne Fleury and Rachel Jeanmaire-Wolf},
  journal={Biology of the cell},
  year={1994},
  volume={81 2},
  pages={95-119}
}
Ciliates are very good models for studying post-translationally generated tubulin heterogeneity because they exhibit highly differentiated microtubular networks in combination with reduced genetic diversity. We have approached the analysis of tubulin heterogeneity in Paramecium through extensive isolation and characterization of monoclonal antibodies using various antigens and several immunization protocols. Eight monoclonal antibodies and 10 hybridoma supernatants were characterized by: i… CONTINUE READING