Isolation and characterization of kikA, a region on IncN group plasmids that determines killing of Klebsiella oxytoca.

@article{Hengen1992IsolationAC,
  title={Isolation and characterization of kikA, a region on IncN group plasmids that determines killing of Klebsiella oxytoca.},
  author={P N Hengen and D Denicourt and V. N. Iyer},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 9},
  pages={3070-7}
}
Transfer of the IncN group plasmid pCU1 from Escherichia coli to Klebsiella oxytoca by conjugation kills a large proportion (90 to 95%) of the recipients of plasmid DNA, whereas transfer to E. coli or even to the closely related Enterobacter aerogenes does not. Two regions, kikA and kikB, have been identified on pCU1 that contribute to the Kik (killing in klebsiellas) phenotype. We have localized the kikA region to 500 bp by deletion analysis and show by DNA-DNA hybridization that kikA is… CONTINUE READING