Isolation and characterization of human muscle cells.

@article{Blau1981IsolationAC,
  title={Isolation and characterization of human muscle cells.},
  author={Helen M. Blau and C U Webster},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1981},
  volume={78 9},
  pages={5623-7}
}
We have developed an in vitro system for the study of postnatal human muscle under standardized conditions. The technique utilizes cloning to isolate pure populations of muscle cells. By manipulating culture conditions we can maximize either proliferation or differentiation of individual clones or of clones pooled to yield mass cultures of muscle cells. The muscle phenotype is stable; cells can be stored in liquid nitrogen for long-term use without loss of proliferative or differentiative… CONTINUE READING

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