Isolation and characterization of human fast skeletal beta troponin T cDNA: comparative sequence analysis of isoforms and insight into the evolution of members of a multigene family.

@article{Wu1994IsolationAC,
  title={Isolation and characterization of human fast skeletal beta troponin T cDNA: comparative sequence analysis of isoforms and insight into the evolution of members of a multigene family.},
  author={Q L Wu and Prakash Kumar Jha and Malay K. Raychowdhury and Y. Du and Paul C. Leavis and Satyapriya Sarkar},
  journal={DNA and cell biology},
  year={1994},
  volume={13 3},
  pages={
          217-33
        }
}
A cDNA encoding human fast skeletal beta troponin T (beta TnTf) has been isolated and characterized from a fetal skeletal muscle library. The cDNA insert is 1,000 bp in length and contains the entire coding region of 777 bp and 5' and 3' untranslated (UT) segments of 12 and 211 bp, respectively. The 3' UT segment shows the predicted stem-loop structure typical of eukaryotic mRNAs. The cDNA-derived amino acid sequence is the first available sequence for human beta TnTf protein. It is encoded by… 
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References

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Characterization of a rabbit fast skeletal troponin I cDNA: a comparative sequence analysis of vertebrate isoforms and tissue-specific expression of a single copy gene.
TLDR
Comparative sequence analysis of the vertebrate TnI isoforms and their cDNAs shows a high level of sequence conservation in the coding region among the TnIf interspecies isoforms, and the sequence similarity is markedly decreased among the fast, slow and cardiac intra- and inter- species isoforms.
Isolation and characterization of cDNA clones encoding embryonic and adult isoforms of rat cardiac troponin T.
  • J. JinJ. Lin
  • Biology, Chemistry
    The Journal of biological chemistry
  • 1989
TLDR
Results suggest that both embryonic and adult isoforms of rat cardiac TnT are generated from the same primary transcript by developmentally regulated alternative splicing.
Amino acid sequence of rabbit cardiac troponin T.
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TLDR
The complete amino acid sequence of the larger, more abundant isoform TnT-1, the thin filament regulatory complex that confers calcium sensitivity to striated muscle contraction and actomyosin ATPase activity, is determined using whole troponin T purified from adult bovine cardiac muscle.
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