Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.

Induction of erythrodifferentiation was carried out by addition of dimenthyl sulfoxide or butyric acid to the culture medium among 80 subclones of T-3-Cl-2 Friend mouse leukemia line. Hemoglobin-positive cells were counted on day 5 of the inducer treatment on the hemocytometer after benzidine staining. Some cell lines were indicible by both of the inducers… CONTINUE READING