Isolation and characterization of heparan sulfate proteoglycans produced by cloned rat microvascular endothelial cells.

@article{Kojima1992IsolationAC,
  title={Isolation and characterization of heparan sulfate proteoglycans produced by cloned rat microvascular endothelial cells.},
  author={Toshiyuki Kojima and Cataldo W Leone and G A Marchildon and J A Marcum and Robert Rosenberg},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 7},
  pages={4859-69}
}
We have isolated heparan sulfate proteoglycans (HSPGs) from cloned rat microvascular endothelial cells using a combination of ion-exchange chromatography, affinity fractionation with antithrombin III (AT III), and gel filtration in denaturing solvents. The anticoagulantly active heparan sulfate proteoglycans (HSPGact) which bind tightly to AT III bear mainly anticoagulantly active heparan sulfate (HSact) whereas the anticoagulantly inactive heparan sulfate proteoglycans (HSPGinact) possess… CONTINUE READING