Isolation and characterization of growth hormone from a marine fish, bonito (Katsuwonus pelamis).

Abstract

Growth hormone (GH) was extracted under alkaline conditions (pH 10) from pituitary glands (6.3 g) of bonito (Katsuwonus pelamis), and subsequently purified by gel filtration, ion exchange chromatography, and reversed-phase HPLC. The GH was monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and by immunoblotting with yellowtail… (More)

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@article{Noso1988IsolationAC, title={Isolation and characterization of growth hormone from a marine fish, bonito (Katsuwonus pelamis).}, author={Toyohiko Noso and A. Yasuda and Ichiro Kawazoe and Hiroki Takehara and Akira Takahasi and K. Sakai and Hirohito Kawauchi}, journal={International journal of peptide and protein research}, year={1988}, volume={32 6}, pages={579-89} }