Isolation and characterization of gonadotropin isohormones from the pituitary gland of pike eel (Muraenesox cinereus).

Abstract

Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol-6% ammonium acetate, pH 5.1 extraction and were purified by DEAE-cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32 P-uptake in 1 day-old chick… (More)

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