Isolation and characterization of genes from the marine microalga Pavlova salina encoding three front-end desaturases involved in docosahexaenoic acid biosynthesis.

@article{Zhou2007IsolationAC,
  title={Isolation and characterization of genes from the marine microalga Pavlova salina encoding three front-end desaturases involved in docosahexaenoic acid biosynthesis.},
  author={Xue-Rong Zhou and Stanley S. Robert and James Robertson Petrie and Dion M. F. Frampton and Maged Peter Mansour and Susan I. Blackburn and Peter David Nichols and Allan G. Green and Surinder Pal Singh},
  journal={Phytochemistry},
  year={2007},
  volume={68 6},
  pages={785-96}
}
The marine microalga Pavlova salina produces lipids containing approximately 50% omega-3 long chain polyunsaturated fatty acids (LC-PUFA) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Three cDNA sequences, designated PsD4Des, PsD5Des, PsD8Des, were isolated from P. salina and shown to encode three front-end desaturases with Delta4, Delta5 and Delta8 specificity, respectively. Southern analysis indicated that the P. salina genome contained single copies of all three front… CONTINUE READING
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