Isolation and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4.

@article{Pinyakong2004IsolationAC,
  title={Isolation and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4.},
  author={Onruthai Pinyakong and Hiroshi Habe and Atsushi Kouzuma and Hideaki Nojiri and Hisakazu Yamane and Toshio Omori},
  journal={FEMS microbiology letters},
  year={2004},
  volume={238 2},
  pages={297-305}
}
Sphingomonas sp. strain A4 is capable of utilizing acenaphthene and acenaphthylene as sole carbon and energy sources, but it is unable to grow on other polycyclic aromatic hydrocarbons (PAHs). The genes encoding terminal oxygenase components of ring-hydroxylating dioxygenase (arhA1 and arhA2) were isolated from this strain by means of the ability to oxidize indole to indigo of the Escherichia coli clone containing electron transport proteins from phenanthrene-degrading Sphingobium sp. strain P2… CONTINUE READING

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