Isolation and characterization of gap junctions from rat liver.

Abstract

Cell to cell communication, through the transmission of small molecules (Mr less than 1200), probably occurs via a membrane specialization, the gap junction. A procedure has been developed for the isolation of gap junctions from rat liver without the use of exogenous proteases. Plasma membranes were prepared from 45 to 50 rat livers (about 500 g) by a… (More)

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