Isolation and characterization of glycosaminoglycans from Schistosoma mansoni.

@article{Robertson1985IsolationAC,
  title={Isolation and characterization of glycosaminoglycans from Schistosoma mansoni.},
  author={N P Robertson and G. D. Cain},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1985},
  volume={82 2},
  pages={299-306}
}
Tegumental tissues of paired adult Schistosoma mansoni were removed by treatment with Triton X-100 and recovered by centrifugation. The chloroform-methanol insoluble residues of this isolated tegumental fraction and of the denuded carcasses were analysed for glycosaminoglycan (GAG) and sialic acid contents. Treatment with GAG-specific enzymes followed by electrophoretic analysis showed that both the carcass and tegument contained heparin and/or heparan sulfate, chondroitin sulfate and… CONTINUE READING