Isolation and characterization of four alternate c-erbB3 transcripts expressed in ovarian carcinoma-derived cell lines and normal human tissues

  title={Isolation and characterization of four alternate c-erbB3 transcripts expressed in ovarian carcinoma-derived cell lines and normal human tissues},
  author={Hakjoo Lee and Nita Jane Maihle},
ErbB-3 is a member of the epidermal growth factor receptor (EGFR/ErbB) family. In addition to the previously reported 6.2 kb full-length and 1.4 kb truncated c-erbB3 transcripts, we have observed a 1.7 kb c-erbB3 human transcript in Northern blots that specifically hybridizes to a probe of the extracellular domain of the receptor. Using 3′-RACE we have isolated four novel c-erbB3 cDNA clones of 1.6, 1.7, 2.1 and 2.3 kb from a human ovarian carcinoma-derived cell line. All four alternate… 

Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes.

It is demonstrated that 3T3-L1 cells express EGFR/erbB1, erbB2 and ErbB3, and possibly, certain erBB2 splice or proteolytic variants, which are worth pursuing.

Expression of erbB-4/HER-4 growth factor receptor isoforms in ovarian cancer.

The use of an anti-erbB4 blocking antibody suggested that erbB4 was not the mediator of the growth stimulatory effects of neuregulin in ovarian cancer cells and indeed could potentially antagonize this effect.

The HER-2/neu receptor tyrosine kinase gene encodes a secreted autoinhibitor.

Herstatin appears to be an inhibitor of p 185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.

Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation

It is demonstrated that expression of a truncated 100 kDa HER2 variant which encodes the extracellular domain of HER2 (HER-ECD) inhibits growth factor-mediated tumour cell proliferation suggesting an important role during the progression of human cancer.

erbB genes in the mouse uterus: cell-specific signaling by epidermal growth factor (EGF) family of growth factors during implantation.

Autophosphorylation and immunoprecipitation studies provided evidence that uterine ErbB3 andErbB4 are biologically active and a comprehensive analysis of possible ligand-receptor signaling schemes for EGF-like ligands in implantation.

ErbB2 and EGFR are downmodulated during the differentiation of 3T3‐L1 preadipocytes

This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation.

Novel ERBB4 juxtamembrane splice variants are frequently expressed in childhood medulloblastoma

An RT‐PCR and sequencing strategy demonstrated the four ERBB4 JM variants to be encoded by two short exons containing the JMb and JMa sequences positioned in the order 5′ to 3′ and separated by a 121 bp intron.




  • Biophys. Res. Commun., 192, 1189 ± 1197.
  • 1993


  • Natl. Acad. Sci. USA, 87, 4905 ± 4909.
  • 1990


  • Natl. Acad. Sci. USA, 89, 7801 ± 7805.
  • 1992


  • Natl. Acad. Sci. USA, 86, 9193 ± 9197.
  • 1989

The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis.

The discovery of restriction endonucleases, which together with the development of DNA ligation and transformation procedures, led to the ability to clone and thus propagate genes of any organism.


  • Natl. Acad. Sci. USA, 88, 1825 ± 1829.
  • 1991

Nature, 348, 693 ± 699

  • Bast RC Jr. (1981). J. Clin. Invest., 68, 1331 ± 1337. Buick R, Pullano R and Trent J. (1985). Cancer Res., 45,
  • 1990


  • J. Cancer, 71, 758 ± 762.
  • 1995


  • Natl. Acad. Sci. USA, 86, 7326 ± 7330.
  • 1989

Cell, 20, 381 ± 390

  • Aroian RV, Koga M, Mendel JE, Ohshima Y and Sternberg
  • 1980