Isolation and characterization of dendritic cells and macrophages from the mouse intestine.

@article{Geem2012IsolationAC,
  title={Isolation and characterization of dendritic cells and macrophages from the mouse intestine.},
  author={Duke Geem and Oscar Medina-Contreras and Wooki Kim and Clifton S Huang and Timothy L Denning},
  journal={Journal of visualized experiments : JoVE},
  year={2012},
  volume={63},
  pages={e4040}
}
Within the intestine reside unique populations of innate and adaptive immune cells that are involved in promoting tolerance towards commensal flora and food antigens while concomitantly remaining poised to mount inflammatory responses toward invasive pathogens. Antigen presenting cells, particularly DCs and macrophages, play critical roles in maintaining intestinal immune homeostasis via their ability to sense and appropriately respond to the microbiota. Efficient isolation of intestinal DCs… CONTINUE READING

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