Isolation and characterization of cytotoxic cyclotides from Viola tricolor

@article{Tang2010IsolationAC,
  title={Isolation and characterization of cytotoxic cyclotides from Viola tricolor
},
  author={Jun Tang and Conan K. Wang and Xulin Pan and He Yan and G. Zeng and Wenyan Xu and Wenjun He and N. Daly and D. Craik and N. Tan},
  journal={Peptides},
  year={2010},
  volume={31},
  pages={1434-1440}
}
Many plants of the Violaceae plant family have been used in traditional remedies, and these plants often contain cyclotides, a particular type of plant cyclopeptide that is distinguished by a cyclic cystine knot motif. In general, bioactive plant cyclopeptides are interesting candidates for drug development. In the current study, a suite of 14 cyclotides, which includes seven novel cyclotides [vitri B, C, D, E, F, varv Hm, and He], together with seven known cyclotides [varv A, D, E, F, H, vitri… Expand
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The Study of Antimicrobial Activities of Partially Purified Cyclotide Content and Crude Extracts from Viola tricolor
TLDR
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References

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The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity.
TLDR
This study shows that the alpine violet Viola biflora (Violaceae) is a rich source of cyclotides and correlates amino acid sequence to cytotoxic potency using a fluorometric microculture cytotoxicity assay using a lymphoma cell line. Expand
A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability.
TLDR
This study confirms that cyclotides may be regarded as a natural combinatorial template that displays a variety of peptide epitopes most likely targeted to a range of plant pests and pathogens. Expand
A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability
TLDR
This study confirms that cyclotides may be regarded as a natural combinatorial template that displays a variety of peptide epitopes most likely targeted to a range of plant pests and pathogens. Expand
Cyclotides: a novel type of cytotoxic agents.
TLDR
Activity profiles of the cyclotides differed significantly from those of antitumor drugs in clinical use, which may indicate a new mode of action in cytotoxic activities of naturally occurring macrocyclic peptides isolated from the two violets. Expand
Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.
TLDR
There is a positive correlation between the hydrophobicity and the anti-HIV activity of the new cyclotides and that this trend tracks with their ability to disrupt membranes, as judged from hemolytic assays on human erythrocytes. Expand
Discovery and characterization of a linear cyclotide from Viola odorata: implications for the processing of circular proteins.
TLDR
The three-dimensional solution structure of violacin A was determined and found to adopt the cystine knot fold of native cyclotides, a naturally occurring linear cyclotide from the plant Viola odorata and the implications for in vivo cyclization of peptides are discussed. Expand
The Cyclotide Fingerprint in Oldenlandia affinis: Elucidation of Chemically Modified, Linear and Novel Macrocyclic Peptides
TLDR
This work increases knowledge about the sequence variation that is accommodated by the cyclic cystine knot scaffold, confirms its applicability as a template for drug design, and shows the first natural degradation pathways for cyclotides. Expand
Cytotoxic cyclotides from Viola tricolor.
TLDR
The most potent compounds isolated, that is, the compounds showing the lowest IC(50) values, were shown to be three small proteins: vitri A, varv A and varv E, of which vitri A is described for the first time. Expand
Biosynthesis and insecticidal properties of plant cyclotides: The cyclic knotted proteins from Oldenlandia affinis
TLDR
A potent inhibitory effect on the growth and development of larvae from the Lepidopteran species Helicoverpa punctigera is described, indicating that the cyclotide domains are excised and cyclized from all four predicted precursor proteins. Expand
Discovery, structure and biological activities of the cyclotides.
TLDR
The background to the discovery of the cyclotides, their structural characterization, chemical synthesis, genetic origin, biological activities and potential applications in the pharmaceutical and agricultural industries are described. Expand
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