Isolation and characterization of cultured human conjunctival goblet cells.

  title={Isolation and characterization of cultured human conjunctival goblet cells.},
  author={Marie A. Shatos and Jos{\'e} David R{\'i}os and Yoshitaka Horikawa and Robin R. Hodges and Eli L. Chang and Carlo R. Bernardino and Peter A. D. Rubin and Darlene A. Dartt},
  journal={Investigative ophthalmology \& visual science},
  volume={44 6},
PURPOSE To isolate and characterize goblet cells from normal human conjunctival tissue to determine whether epidermal growth factor (EGF) receptors are present and whether EGF can influence goblet cell proliferation. METHODS Goblet cells were isolated from explant cultures established from normal conjunctival tissue harvested from patients during periocular surgery. The cells were grown in RPMI culture medium supplemented with 10% fetal bovine serum and characterized using morphology… 

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