Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara.

@article{Senis2006IsolationAC,
  title={Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara.},
  author={Yotis A Senis and Paul Y Kim and Gemma L J Fuller and Angel Turegano Garc{\'i}a and Sripadi Prabhakar and M. C. Wilkinson and Helen Brittan and Nicole Zitzmann and Robin Wait and David A. Warrell and Stephanie Watson and Aura S. Kamiguti and Robert David Geoffrey Theakston and Michael E. Nesheim and G Laing},
  journal={Biochimica et biophysica acta},
  year={2006},
  volume={1764 5},
  pages={863-71}
}
In this study, we isolated a novel prothrombin activator from the venom of Bothrops cotiara, a Brazilian lance-headed pit viper (Cotiara, Jararaca preta, Biocotiara), which we have designated "cotiaractivase" (prefix: cotiar- from B. cotiara; suffix: -activase, from prothrombin activating activity). Cotiaractivase was purified using a phenyl-Superose hydrophobic interaction column followed by a Mono-Q anion exchange column. It is a single-chain polypeptide with a molecular weight of 22,931 Da… CONTINUE READING