Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking.

@article{Motaleb2007IsolationAC,
  title={Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking.},
  author={Md Abdul Motaleb and Michael R. Miller and Richard G Bakker and Chunhao Li and Nyles W. Charon},
  journal={Methods in enzymology},
  year={2007},
  volume={422},
  pages={421-37}
}
Constructing mutants by targeted gene inactivation is more difficult in the Lyme disease organism, Borrelia burgdorferi, than in many other species of bacteria. The B. burgdorferi genome is fragmented, with a large linear genome and 21 linear and circular plasmids. Some of these small linear and circular plasmids are often lost during laboratory propagation… CONTINUE READING